Laboratory of Plant Health and Microbiology
E-mail taimetervis[a]pmk.agri.ee, Phone +372 672 9161
Analysis and activities conducted:
- Bacterial diseases and phytoplasmas on plant material:
potato ring-rot and potato brown rot, fire-blight on fruit trees and strawberry leaf spot disease, Xylella fastidiosa etc.
- Plant viruses and viroids on:
potatoes (PVA, PVY, PVX, PVM, PVS, PLRV, and PSTVd); vegetables (PepMV, TSWV, CMV); fruit trees (PPV); berry trees (BRV, BRRV); ornamental plants (HVX) etc.
- Plant and soil nematodes and nematodes on wood:
potato cyst nematode, potato tuber nematode, stem and bulb eelworm, strawberry foliar nematode, pine wood nematode etc.
- Insect pests, mites, fungal diseases from plants, plant products, and detection of weed seeds
- Identification and confirmation of plant diseases and pests by molecular biology methods and sequencing
- Microbiological analyses of plant products, feedstuff, compost and soil
- Bacteria, aerobic sporulation bacteria, pathogenic bacteria, yeasts and moulds, Fusarium sp from agricultural products:
cereal, silage, hay, feedstuff, feedstuff components etc.
- Detection of general toxicity in cereal, cereal products, feedstuffs and feedstuff components
- Preserving and expanding the collection of micro-organisms necessary for applied researc
Price list of Laboratory of Plant Health and Microbiology
Prices for 2022
|
Name of analysis/service |
Price, € |
|
|
|
BACTERIOLOGICAL ANALYZES |
|
|
1 |
Potato ring rot (Clavibacter sepedonicus) per sample of 200 tubers c |
84.00 |
|
2 |
Potato brown rot (Ralstonia solanacearum) per sample of 200 tubers c |
84.00 |
|
3 |
Fire Blight (Erwinia amylovora) |
77.00 |
|
4 |
Apple proliferation phytoplasma (Candidatus phytoplasma mali) and Pear decline phtytoplasma (Candidatus phytoplasma pyri) by PCR-method - 1 sample |
114.00 |
|
5 |
Apple proliferation phytoplasma (Candidatus phytoplasma mali) and Pear decline phtytoplasma (Candidatus phytoplasma pyri) by PCR-method - monitoring a batch of 50 samples |
569.00 |
|
6 |
Zebra chip disease (Candidatus liberibacter solanacearum) by PCR-method - per sample |
102.00 |
|
7 |
Other bacteriological analysis by inoculation, IF- or PCR methods and sequencing a - per sample |
97.00 |
|
VIROLOGICAL ANALYZES b |
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|
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By ELISA-method: |
|
|
|
8 |
Potato virus 1 sample 110 tubers - basic price |
75.00 |
|
9 |
Potato virus 1 sample 110 tubers - 1 virus |
110.00 |
|
10 |
Potato virus 1 sample 110 tubers in 4 groups - basic price |
58.00 |
|
11 |
Potato virus 1 sample 110 tubers in 4 groups - 1 virus |
58.00 |
|
12 |
6 potato viruses (PVY, PVA, PVX, PVM, PVS, PLRV) from the same sample, per sample of 110 tubers divided into groups of 4 |
403.00 |
|
13 |
One plant virus, monitoring up to 77 samples |
214.00 |
|
By Real-Time -RT-PCR-method: |
|
|
|
14 |
Plant virus, 1 sample - basic price |
35.00 |
|
15 |
Plant virus, 1 sample - 1 virus |
48.00 |
|
16* |
Plant virus up to 10 samples - base price |
46.00 |
|
17* |
Plant virus up to 10 samples - 1 virus |
68.00 |
|
18* |
Plant virus up to 20 samples - base price |
88.00 |
|
19* |
Plant virus up to 20 samples - 1 virus |
108.00 |
|
20 |
Plant virus up to 50 samples - 1 base price |
231.00 |
|
21 |
Plant virus up to 50 samples - 1 virus |
124.00 |
|
22 |
One potato virus directly from the tuber, 1 sample 100 tubers divided into groups of 10 |
53.00 |
|
23 |
One additional virus from the same sample, 1 sample 100 tubers divided into groups of 10 |
54.00 |
|
24 |
Pepino Mosaic Virus (PepMV) from tomato seeds, 1 per sample |
118.00 |
|
By PCR/RT-PCR method: |
|
|
|
25 |
Pospiviroid - per sample (except the CLV) |
132.00 |
|
26 |
Plant virus, 1 sample - basic price |
35.00 |
|
27 |
Plant virus, 1 sample - 1 virus |
61.00 |
|
28 |
Plant virus up to 10 samples - base price |
70.00 |
|
29 |
Plant virus up to 10 samples - 1 virus |
100.00 |
|
30 |
Plant virus up to 20 samples - base price |
137.00 |
|
31 |
Plant virus up to 20 samples - 1 virus |
159.00 |
|
32 |
Plant virus up to 50 samples - 1 base price |
239.00 |
|
33 |
Plant virus up to 50 samples - 1 virus |
298.00 |
|
34 |
Plant viruses with multiplex method: CMV, INSV, TSWV, AMV and HVX, per sample |
100.00 |
|
35 |
Plant viruses with multiplex method: CMV, INSV, TSWV, AMV and HVX, up to 10 samples |
200.00 |
|
ENTOMOLOGICAL ANALYZES |
|
|
|
36 |
Entomological analysis (Dangerous pests) - per sample |
77.00 |
|
37 |
Entomological analysis (common house and garden pests) - per sample |
53.00 |
|
38 |
GLUE TRAP (1 trap = insects from same species) |
103.00 |
|
39 |
Entomological analysis by PCR and sequencing - per sample |
110.00 |
|
NEMATOLOGICAL ANALYZES |
|
|
|
40 |
Cyst nematodes from plant material, including potato cyst nematodes from potato tubers, soil and peat (Globodera pallida, Globodera rostochiensis) (1 soil sample / peat sample 400 ml) |
59.00 |
|
41 |
Free-living nematodes from plant tissues, leaves, buds, stems, soil and peat (pine wood nematode, potato tuber nematode, stem and bulb eelworm etc) - per sample |
63.00 |
|
42 |
Meloidogyne fallax and Meloidogyne chitwoodi from potato tubers (sample of 200 tubers) |
110.00 |
|
43 |
Pine wood nematode from longhorn beetles body (included identification of longhorn beetles to species). Entomological + nematological analysis |
76.00 |
|
44 |
Nematological analysis by PCR methods and sequencing - per sample |
96.00 |
|
MYCOLOGICAL ANALYZES |
|
|
|
45 |
Karnal bunt of wheat (Tilletia indica)- per sample |
53.00 |
|
46 |
Potato wart disease (Synchytrium endobioticum) from the soil - per sample |
97.00 |
|
47 |
Sudden oak death (Phytophthora ramorum) - per sample |
87.00 |
|
48 |
Phytophthora kernoviae - per sample |
80.00 |
|
49 |
Pitch canker (Gibberella circinata; Fusarium circinatum) - per sample |
108.00 |
|
50 |
Dothistroma blight (Mycosphaerella pini) or brown spot needle blight (Mycosphaerella dearnessii) - per sample |
70.00 |
|
51 |
Other mycological analysis by biomethod and/or microscoping - per sample |
110.00 |
|
52 |
Mycological analysis by PCR methods and sequencing - per sample |
110.00 |
|
53 |
Weed detection - per sample |
38.00 |
|
54 |
Primary detection of plant diseases and pests - per sample |
47.00 |
|
MICROBIOLOGICAL ANALYZES |
|
|
|
55 |
Butyric acid bacteria (Clostridium spores) |
15.70 |
|
56 |
Denitrifying bacteria on Hiltay medium |
16.80 |
|
57 |
Yeasts and moulds (total count) |
14.70 |
|
58 |
Total bacterial count |
11.00 |
|
59 |
Aerobic sporulating bacteria (Bacillus spp. spores) |
14.70 |
|
60 |
Fusarium sp on Nash and Snyder medium |
17.80 |
|
61 |
Actinomycetes on starch-ammonium agar |
22.00 |
|
62 |
Azotobacter on Ashby medium |
22.00 |
|
63 |
Sulfate reducing bacteria on Starkey medium |
18.00 |
|
64 |
Lactic acid bacteria (Lactobacillusspp.) |
13.30 |
|
65 |
Nitrifying bacteria on water agar |
24.50 |
|
66 |
Cellulose degrading bacteria on Hutchinsoni medium |
21.00 |
|
67 |
Enterobacteriaceae |
14.00 |
|
68 |
Coliform bacteria |
13.00 |
|
69 |
Escherichia coli |
13.60 |
|
70 |
Salmonella spp |
18.00 |
|
71 |
Listeria monocytogenes |
23.70 |
|
72 |
Clostridium perfringens |
19.00 |
|
73 |
Determination of the toxicity with the Bacillus stearothermophilus |
17.80 |
|
74 |
General toxicity (with infusoria) |
24.00 |
|
75 |
Determination of impurities, stones and maximum size of particles from sludge, treated biowaste and soil |
45.00 |
|
76 |
Other services (according to detailed calculation) |
|
|
77* |
Identification of field crop varieties using molecular markers (2 markers) d |
255.00 |
|
78* |
Identification of field crop varieties using molecular markers (5 markers) d |
466.00 |
|
79* |
Identification of field crop varieties using molecular markers (10 markers) d |
833.00 |
|
80* |
Sample handling (for transfer to another laboratory for analysis at the customer's request) |
17.00 |
|
Issue of technical culture |
|
|
|
81 |
Technical culture on liquid medium EUR/l (wine yeast, Rhizobium culture etc) |
39.00 |
|
82 |
Technical culture on solid medium EUR/piece (moulds, yeasts, bacteria) |
20.00 |
|
83 |
Pleurotus ostreatus - fungi culture on the grain EUR/piece |
23.00 |
|
84 |
Test Report on paper |
3.60 |
|
* New service |
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Appendix a |
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Other bacteriological analysis by inoculation, IF- or PCR methods and sequencing: incl. Xylella fastidiosa, Pseudomonas syringae pv. actinidae, Pseudomonas syringae pv. aesculi |
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|
Appendix b |
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Plant viruses by Real-Time-RT-PCR-method: Tobacco Rattle virus (TRV), Potato Mop-Top virus (PMTV), potato viruses (PVY, PVA, PVM, PVX, PVS, PLRV), Pepino Mosaic Virus (PepMV), viroids (Potato spindle tuber viroid (PSTVd), Columnea latent viroid (CLVd) |
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|
Plant viruses by ELISA-method: Pepino Mosaic Virus (PepMV), Plum Pox Virus (PPV), Tomato Spotted Wilt Virus (TSWV), Impatiens Necrotic Spot Virus (INSV), Cucumber Mosaic Virus (CMV), Alfaalfa Mosaic Virus (AMV), Potato Mop Top Virus (PMTV), Hosta Virus X (HVX), potato viruses (A, S, M, X, Y, PLRV) |
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|
Plant viruses by PCR/RT-PCR method: Cucumber Mosaic virus (CMV), Impatiens Necrotic Spot virus (INSV), Tomato Spotted Wilt virus (TSWV), Alfalfa Mosaic virus (AMV), Hosta virus X (HVX), Black Currant Reversion virus (BRV), Plum Pox Virus (PPV), Pepino Mosaic Virus (PepMV), Tomato Leaf Curl New Delhi Virus (ToLCNDV), Blueberry red ringspot virus (BRRV), viroids (Potato spindle tuber viroid (PSTVd), Columnea latent viroid (CLVd), Chrisanthemum stunt viroid (CSVd) and so. |
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|
Plant viruses by RT-PCR multiplex method: CMV, INSV, TSWV, AMV and HVX |
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Appendix c |
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From the same sample of potato tubers it is possible to detect potato ring-rot, potato brown-rot and quality viruses |
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Appendix d |
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Before ordering the analyse "Identification of field crop varieties using molecular markers" please consult with laboratory if desired varietis are possible to analyse and if so then with how many markers. |
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